vialdev

I think you guys overestimate how fragile these sequences are for typical bench use. Bacteriostatic water keeps things stable enough as long as you avoid direct sunlight and keep the vial clean.

benchroom

You are worrying too much about minor degradation unless you are running months long multi phase studies. Most research subjects complete their protocols well before any noticeable drop off occurs in a standard fridge.

kineticmind

From what I have seen in stability studies on similar molecules aqueous solutions degrade way faster than lyophilized powder due to deamidation and oxidation. Storing them at room temp for even a few days is basically asking for half life reduction.

echo88

That is a fair point about the total timeline being short anyway but I am chasing absolute precision for my current log. Even a five percent potency drift bothers my analytical side.

cyclefox

Exactly my thoughts. Chemical stability is a totally different beast compared to sterility. That is why I always try to source fresh batches and discard anything sitting past the fourteen day mark.

quietx74

Bacteriostatic water helps prevent microbial growth sure but it does nothing to stop chemical hydrolysis over time. The solvent itself can drive degradation pathways if left too long.

molarstack35

If you want absolute precision you should be freezing aliquots right after reconstitution. Keeping liquid solutions in a standard fridge past two weeks always shows some minor secondary peaks on the chromatography readout in my experience.

ambernotes

Freezing and thawing cycles can cause shear stress on larger peptide chains though so you have to be careful with that approach too. It is a constant trade off between oxidation at fridge temps and structural damage from ice crystal formation.