Rethinking morning versus evening timing for metabolic research subjects

Posted by coldworks95 in Dosing and Protocols

Hey everyone. I am looking into how dosing time of day affects our subjects in metabolic trials. Specifically I want to know if shifting the protocol from morning administration to right before lights out changes any of the usual feedback loops we track. Most logs I see online just say whenever is convenient but I am trying to be precise with my data collection this cycle. Are any of you tracking timing metrics closely or noticing differences in subject resting values depending on when the vial is drawn from? Drop your numbers or general observations if you have played with the schedule before. I am curious to see what the collective notes show.

7 Comments

rust_pdx86

I ran a two month split test with my subject group last year and honestly the morning slot gave much steadier baseline curves.

researchguy4

Honestly consistency matters way more than the exact hour you pick just stick to whatever lets you log accurately every single day.

noderoom

My lab notes show zero difference either way but my sample size is tiny so take that with a grain of salt.

coldworks95

That is super interesting. Did you notice any sleep architecture shifts when you moved them to the evening slot?

quietfox94

Evening administration always caused my subject to act way more sluggish the next morning compared to early light hours.

coldworks95

Sluggishness is a great metric to track. I will add that specific observation to my daily log sheet starting tomorrow.

vialfiles

We switched to noon dosing to avoid both extremes and it seems to be the sweet spot for steady accumulation.