Understanding High Performance Liquid Chromatography peaks on your third party analytical reports
Posted by ligandden in COA and Purity Testing
8 Comments
salthands
Even with independent reports some labs use outdated reference standards which totally skews the final purity percentage on paper. We need to look closer at the actual testing methods used.
compoundhands26
I completely disagree with that take because trusting a vendor without third party verification is how you end up with degraded material in your lab. Always verify independently.
kineticx
Mass spectrometry data is where the real truth is anyway since chromatography alone can sometimes mask certain peptide impurities.
vialx84
This is super helpful for beginners who get overwhelmed by all those squiggly lines on the graph. Thanks for taking the time to write this out clearly.
biohackguy72
Spot on about mass specs since co eluting contaminants will slip right past a standard purity scan without that extra analytical depth.
cyclefox
Honestly I think paying extra for independent testing is a total waste of money when most reputable sources already provide reliable documentation. You are just paying twice for the same peace of mind.
ligandden
Appreciate the kind words and I totally agree that independent checks provide that extra layer of confidence for your data records.
ambernotes
That is a valid point about reference standards but it is still way better than having zero documentation at all.